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However, when cells were transferred to culture medium without added iron ( Fe), expression of the pcbB gene was activated, a surprising result as pcb genes of Prochlorococcus were not known to be regulated by iron as is the iron stress induced isiB gene (Table 1). The latter gene encodes flavodoxin12, which substitutes for ferredoxin as an electron acceptor to PSI, and its expression indicates that the cells had acclimatized to conditions of iron depletion. On the other hand, the expression of the pcbA as well as the psaA and psbA genes, encoding PSI and PSII reaction centre proteins, respectively, were downregulated (Table 1).Table 1:Quantitative RT analyses of the expression of the indicated genes under and Fe conditionsFull tableThe question arising from these expression studies is: where is the PcbB protein targeted in cells exposed to low iron levels? As before, thylakoid membranes were isolated, solubilized and subjected to sucrose density gradient centrifugation.
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